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Robert Hondal, Ph.D.
Associate Professor
Robert.Hondal@uvm.edu
O: B413 Given L: B413 Given
O: 802-656-8282 L: 802-656-8294

Research Interests:
Cancer Biology
Enzymology
Structural Biology & Protein Function

Background:
Dr. Hondal received his Ph.D. in Chemistry from the Ohio State University in 1997. He then spent one year in the laboratory of Raymond Burk at Vanderbilt University studying the chemistry of selenomethionine and the structure of selenoprotein P. He continued his postdoctoral study under the direction of Ronald Raines at the University of Wisconsin-Madison as a NIH Postdoctoral Fellow. His work there focused on state of the art protein engineering techniques, including native chemical ligation and expressed protein ligation. His postdoctoral work at UW-Madison focused on methods for inserting non-natural amino acids into proteins as a means of addressing important structure-function questions in enzymology.

 

Project Description:

There are two main areas of research in my lab. First, we are concerned with the mechanistic enzymology of high molecular weight thioredoxin reductases. The mammalian form of this enzyme contains the rare amino acid selenocysteine. One focus of this project is to understand why the mammalian enzyme requires this rare amino acid, while other homologues from C. elegans and D. melanogaster contain a conventional cysteine residue instead. We use protein engineering and protein semisynthesis techniques to study these enzymes. Semisynthesis allows us to insert a number of non-natural amino acids into the enzyme, as well as produce the mammalian form of the enzyme containing selenocysteine. X-ray crystallography, NMR spectroscopy, peptide synthesis, and steady-state kinetics are all techniques emphasized in my laboratory.

A second project involves developing new methods for regioselective disulfide bond formation in peptides and small proteins. A large challenge for peptide chemists is correctly pairing half-cystinyl residues. This is achieved through orthogonal protection/deprotection schemes using multiple protecting groups for the sulfhydryl group of cysteine. These groups are then selectively removed one at a time to form the correct disulfide bond.

 
Selected Publications:

Eckenroth, BE, Lacey, BM, Lothrop, AP, Harris, KM, and Hondal RJ (2007) Investigation of the C-termindal Redox Center of High Mr Thioredoxin Reductases by Protein Engineering and Semisynthesis. Biochemistry In Press

Eckenroth, BE, Rould, MA, Hondal,RJ, and Everse SJ (2007) Structural and Biochemical Studies Reveal Differences in the Catalytic Mechanisms of Mammalian and Drosophila melanogaster Thioredoxin Reductases. Biochemistry 46, 4694-4705

Harris, KM, Flemer, S, and Hondal RJ (2007) Studies on deprotection of cysteine and selenocysteine side chain protecting groups. J. Pept. Sci 13, 81-93

Eckenroth BE, Harris, K, Turanov, AA, Gladyshev, VN, Raines, RT, and Hondal, RJ (2006) Semisynthesis and characterization of mammalian thioredoxin reductase Biochemistry 45, 5158-5170.

Ruggles, EL, and Hondal, RJ (2006) Synthesis and properties of disulfide-bond containing eight-membered rings. Tet. Lett. 47, 4281-4284

Lacey, BM and Hondal, RJ (2006) Characterization of Mitochondrial Thioredoxin Reductase from C. elegans. Biochem. Biophys. Res. Commun. 346, 629-636.

Hondal, RJ, & Raines, RT (2002) Semisynthesis of Proteins Containing Selenocysteine. Methods Enzymol. 347, 70-83

All Hondal publications